Genome Mapping

In a long-term study commenced in 1997, an advanced population of narrow-leaf lupin (Lupinus angustifolius L.) recombinant inbred lines (RILs) has been developed from a cross between a domesticated and a wild-type parent.  In this RIL population, a number of key domestication traits are segregating, including: mollis (soft seeds); leucospermus (white flower and seed colour); lentus (reduced pod-shattering); iucundis (low alkaloid); and Ku (early flowering), as well as the anthracnose resistance gene, Lanr1.

The RIL mapping population is housed at DAFWA and there is limited seed available for the wider lupin genetic research community, which can be obtained by contacting Dr. H. Yang ([email protected]).

1. On the basis of genetic segregation of marker alleles and the domestication traits in this population, the first genome map based on 89 RILs was constructed by Boersma et al. (2005) using MFLP, a marker technology developed by CLIMA researchers from DAFWA and UWA.  The 2-page PDF (lupinmap1.pdf) below illustrates the genetic linkage mapping framework developed.

2. A gene-based marker map based on 93 RILs was subsequently developed by Nelson et al. (2006) as part of a multi-institutional collaboration.  This map was compared to the partially-sequenced legume genome Medicago truncatula.  Despite the wide taxonomic distance between these legume species, there was still evidence of conserved synteny between the two species.  Another outcome of the second map was the confirmation that the lupin genome underwent duplication in the distant past, probably by a process of polyploidisation.

A lupin mapping database is hosted by Legume DB (https://ccg.murdoch.edu.au/cmap/legumedb/).

An integrated map combining both mapping data sets is under development and will also be hosted at the above site.

For more information, contact:

Mr Jeff Boersma:[email protected]

Dr H. Yang:[email protected]

Matthew Nelson[email protected]

References:
Boersma J, Pallotta M, Li C, Buirchell B, Sivasithamparam K, and Yang H (2005). Construction of a genetic linkage map using MFLP, and identification of molecular markers linked to domestication genes in narrow-leafed lupin (Lupinus angustifolius). Cellular and Molecular Biology Letters 10: 331-344.

Nelson M, Phan H, Ellwood S, Moolhuijzen P, Bellgard M, Hane J, Williams A, Fosu-Nyarko J, Wolko B, Ksi¹¿kiewicz M, Cakir M, Jones M, Scobie M, O’Lone C, Barker S, Oliver R, Cowling W (2006).  The first gene-based map of Lupinus angustifolius L. - location of domestication genes and conserved synteny with Medicago truncatula.  Theoretical and Applied Genetics (DOI: 10.1007/s00122-006-0288-0).

LupinMap.pdf
Genetic linkage mapping framework in narrow-leafed lupin. [PDF 269 KB] Updated 13 Sep 2005 14:55 Size: 325.7 kb