Markers for Phomopsis Resistance
There are at least three major genes in Lupinus angustifolius conferring resistance to phomopsis blight disease. The following two molecular markers are linked to the gene “Phr1”, which is present in Australian breeding line 75A:258. For more information, please contact Dr. H. Yang
[email protected]Sequence of marker “Ph258M1”:
Resistance allele: GGGAACAACA ACAACAAC-- --CAGTTCAAT TCTGGTACTG
Susceptible allele: GGGAACAACA ACAACAACAA CCAGTTCAAT TCTGGTACTG
AACATTATGT TCAGCAGAAC CAGCATCAAC AACAACATTA TGGTGTTCCT
AACATTATGT TCAGCAGAAC CAGCATCAAC AACAACATTA TGGTGTTCCT
CAGAAAAGCT ATGCTGGGGA CAGTGTTGTT CCAGTTCAGC AACACCAGCA
CAGAAAAGCT ATGCTGGGGA CAGTGTTGTT CCAGTTCAGC AACACCAGCA
AGGACATCAT TTTCAACTTG GGTTGGAGTT TGAATCACCC AAAGCTGGAT
AGGACATCAT TTTCAACTTG GGTTGGAGTT TGAATCACCC AAAGCTGGAT
TTAGTTACAA TGGTTCTATT AGTCAAAGTG TAAGTTTCTT CAATCACTAC
TTAGTTACAA TGGTTCTATT AGTCAAAGTG TAAGTTTCTT CAGTCACTAC
TTTTTTTTTG TGATTAA
TTTTTTTTTG TGATTAA
Sequence of marker “Ph258M1”:
Resistance allele: CAGGCACATA TATCTTTATA CCTTCCCTCA TAATACCCTT
Susceptible allele: CAGGCACATA TATCTTTATA CCTTTCCTCA TAATACCCTT
AGACCTTGTT GACTACACCT CAGGCACATA ATTCTCAGCT GTGGCATCCG
AGACCTTGTT GACTACACCT CAGGCACATA ATTCTCAGCT GTGGCATCCG
GATTCTGGTG CCACGCATCA TCTAACTGCT GATCCTATGA ATTCATGCAA
GATTCTGGTG CCACGCATCA TCTAACTGCT GATCCTATGA ATTCATGCAA
AGGTCTGAGC TGTTGTGTGG TCAGGACCAT ATTTATGGGC AATGATATTG
AGGTCTGAGC TGTTGTGTGG TCAGGACCAT ATCTATGGGC AAT -- -- --
TCGCTACCCC TTTTTTACCC CGGTCAAAGT TGGGTGTTTA CAGATATAGG
--- ------ --- --- --- --- --- --- --- --- -- GATATAGG
TATTCCCATT ACTTTTTCCA ACTTCCAACT TCTATCTATT ACTAAGAATA
TATTCCCATT ACTTTTTCCA ACTTCCAACT TCTATCTATT ACTAAGAATA
TAGTCAGTCT GGA
TAGTCAGTCT GGA
Yang, H., Shankar, M., Buirchell, B.J., Sweetingham, M.W., Caminero, C. and Smith, P.M.C. 2002. Development of molecular markers using MFLP linked to a gene conferring resistance to Diaporthe toxica in narrow-leafed lupin (Lupinus angustifolius L.) Theor. Appl. Genet. 105: 265-270.
University information
CRICOS Code: 00126G
